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Silica Coated Gold Nanoparticles

*VAT and shipping costs not included

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Gold nanoprisms could be appropriate candidates for in vitro cellular thermoablation due to their efficient internalization and heating capacity

 CharacterizationServices

 

 

The physical and chemical properties of magnetic nanoparticles largely depend on the synthesis method and chemical structure. The 25nm magnetic nanoparticles are highly monodisperse nano-octahedrons of magnetite capped with citrate anions. These nanoparticles can be employed as platforms for many applications such as magnetic separation, hyperthermia, biosensors.

CITRATE STABILIZED:

Attachment of (bio)molecules containing thiols groups –SH.

CARBOXILATED :

Can be activated by EDC and NHS to form an intermediate that can subsequently react with primary amine groups on a specific protein or any other ligand to be conjugated.

OCTAHEDRAL

APPLICATIONS:

> Magnetic separation
> Biosensing
> Magnetic hyperthermia
> Drug delivery

If your company needs to increase its medical device portfolio or have discover a specific biomarker that needs to be detected in very low quantities, we will be happy to introduce you our device.
heatsens_codevelopment

For more information:

> Visit the project specific website: heatsens.com

> Contact: sales@nanoimmunotech.es

Along these years, we have developed a bioconjugation knowhow to provide our customers the best solution for their projects, meaning that nowadays we also give you the possibility to design multifunctional structures for more accurate outcomes.

  Heatsens_techology

 

These biosensors can be applied to a broad range of sectors and applications, from the development of point-of-care devices for their use in a hospital o directly at the patient home in the clinical diagnosis field, to its use in a chicken farm for the detection of pathogenic microorganisms, or the detection of contaminants in water, and much more.

 Heatsens_sectors

Nanoimmunotech offers different potential formats of HEATSENS® technology for diverse customer needs.

 

Biodistribution, acute toxicity and maximum tolerated dose (OECD 423). Necropsy report of animals and collection of organs showing abnormalities.

Measurement of the immune response IgG and IgM to a foreign antigen in rats treated daily with or without a sample. Histological report of organs, blood chemistry and hemogram.

Determination of contact properties of the nanomaterials with blood cells or plasma proteins.

Coagulation: intrinsic, extrinsic and common coagulation pathways are assessed by partial thromboplastin time (APTT) prothrombin time (PT) and Thrombin time (TT), respectively. Based on free interference Viscosity Detection System (VDS).

Haemolysis: colorimetric measurement of haemoglobin released.

Platelet activation: activated platelets detected by flow cytometry and aggregation by microscopy.

Immunomodulation in PBMCs (peripheral blood mononuclear cells), macrophages and cell lines.

Phagocytosis: Functional evaluation and microscopy monitoring of nanoparticle internalization by phagocytic cells.

Inflammasome: Caspase-1 activation by flow cytometry and induction of IL-1β by enzyme immunoassay (ELISA).

Leukocyte proliferation: Clonal proliferation of PBMCs measured by flow cytometry.

Immunological response: Inflammatory cytokine profile and Th1, Th2, Th17 response of PBMCs measured by flow cytometry or Luminex multiplex technology.

Complement activation: Identification in serum of C3/C3b by western-blot. Quantification of C3b by enzyme immunoassay (ELISA).

 

Selection of suitable cell lines according to the administration route, biodistribution and expected clearance.

Cell viability assay: MTS in metabolically active cells as indicator of proliferation. Discrimination of viable and non‐viable cells using propidium iodide and trypan blue. xCELLigence system for label-free and real-time monitoring of cell -viability.

Apoptosis: Annexin V / propidium iodide, caspase 3 and caspase 8 activation by flow cytometry Reactive Oxygen Species: determination of reactive oxygen species by flow cytometry.

Quantification of colony-forming units of aerobic bacteria, yeast and mold.

Quantification of endotoxin units with different methods based on the use of Limulus Amebocyte Lysate -LAL. Preliminary screening, quantification, inhibition and enhancement.

Determination of the colloidal stability and aggregation in biological media such as saline solution, culture media, cell extracts, whole-blood or serum. 

Microscopy techniques:

Size, shape, monodispersity and composition:
- Transmission Electron Microscopy (TEM).
- Scanning Electron Microscopy (SEM).

 

Elemental Analysis:

Determination of the mass fractions of carbon (C), hydrogen (H), nitrogen (N), and sulfur (S).

 

Nuclear magnetic resonance spectroscopy (NMR):

Molecular structure determination.

 

Inductively coupled plasma (ICP):

Identification and quantification of chemicals elements.

 

Zeta potential:

Measurement of surface charge of nano/microparticles in solution.

 

Dynamic light scattering (DLS):

Hydrodynamic radius and size distribution of nanoparticles in solution.

 

Fourier transform infrared spectrophotometry (FTIR):

Characterization of the major functional groups present on the surface of the sample.

X-ray photoelectron spectroscopy:

Assessing the nature and chemical state of the surface atoms of the nanostructure to depth of 5 nm (4-20 atomic layers).

 

Fluorescence spectrophotometry:

Fluorescence analysis of samples in solution.

 

Superconducting Quantum Interference Devices (SQUID):

Determination of magnetic properties of nanostructures.

 

UV-visible spectroscopy:

Quantitative analysis of analytes.

 

Total reflection x-ray fluorescence analysis (TXRF):

Qualitative and quantitative analysis of chemical elements in solid and liquid samples.

 

Thermogravimetric analysis (TGA):

Determination of the amount of weight change of a material as a function of increasing temperature in an atmosphere of nitrogen or air.

 

Mass spectrometry (MS):

Determination of the mass of molecules.

APLICATIONS

> Biosensors design
> Surfaces functionalization
> Chips biofunctionalization
> Lateral flow test
> Magnetic separation

 

 

 

 

This kit is thought to easily modify your antibodies with oligonucleotides with length varying between 10 and 100 bases preserving their biological activity. With OligoLink kit ANTIBODY you will obtain highly-purified oligonucleotide-antibody conjugates in easy and rapid way, ready to be used in a broad range of applications such as ImmunoPCR, flow cytometry, western blotting, ELISA, etc

APLICATIONS

> Immuno-PCR
> PLA (Proximity Ligation Assay)
> PEA (Proximity Extension Assay)
> ECPA (Electrochemical Proximity Assay)

 

 

 

 

Easy-to-use magnetic nanoparticle conjugation Kit for proteins. The kit guarantees the covalent immobilization of a protein of your own choice to carboxylic groups of 200 nm magnetic nanoparticles (MNPs).

APLICATIONS

> Magnetic separation
> Immunoprecipitation
> Biosensors

 

Easy-to-use magnetic nanoparticle conjugation kit for antibodies. The kit guarantees the ORIENTED covalent immobilization of IgG antibodies of your own choice to 200 nm magnetic nanoparticles (MNPs).

APLICATIONS

> Magnetic separation
> Immunoprecipitation
> Biosensors

 

 

The all-in-one solution for an easy conjugation of your oligonucleotides (10 and 100 bases) to high quality nanoparticles. This kit guarantees the ORIENTED conjugation of your oligonucleotide to high quality gold nanoparticles via a thiol group chemically introduced to either its 5´- or 3´-end.

 

APLICATIONS

> Probes in DNA-based biosensing assays 

First kit that allows ORIENTED conjugation of antibodies and his-tagged recombinant proteins on gold nanoparticles, on an easy and quick way. You will achieve better results than the ones obtained with other conjugation methods.

ORIENTED Bioconjugation of antibodies allows maximal antigen binding and favourable protein orientation meaning no loss of their biological activity. Our method also avoids nonspecific bindings increasing kit yield and sensibility in your assay.

Aplications

> Lateral flow
> Design of biosensors
> Bioconjugation
> Microscopy probe
> Immunogold

 

Magnetic particles on the nanoscale behave as superparamagnetic leading to particles with much higher magnet susceptibilities than in traditional paramagnets. We have developed unique magnetic nanoparticles in the market, covering them with gold, providing all the properties of the gold (inertness, protection against oxidation, easy to conjugate) besides to magnetism.

 image027

Our Magnetic nanoparticles coated with gold are available in sizes ranging from 50 nm to 250 nm in diameter with different surface functionalities in a variety of solvent compositions.

CITRATE STABILIZED:

Gold shell is coated with citrate anions which can be displaced with a wide range of molecules containing a thiol (SH) group.

COOH-PEG:

Gold shell is functionalized with poly(ethylene glycol)–thiol (PEG–SH) ligands (3000 and 5000 Da) having a carboxylic acid end group, which can be further used to immobilize covalently biomolecules by formation of stable amide bonds with primary amines.

 

Aplications

> Magnetic separation
> Magnetic hyperthermia
> Biosensors
> Flow cytometry
> Drug delivery
> Lateral flow tests
> Cancer photothermal therapy
> Conductors in electronic chips

 

We offer Silver nanoparticles that can be used in applications related to their anti-microbial properties, high electrical conductivity, and optical properties. We produce the smallest PEGylated silver nanoparticles in the market

image024 image025

Our Gold nanoparticles are available in sizes ranging from 20 nm to 35 nm in diameter with COOH-PEG surface functionalization:

  • COOH-PEG: Covalent attachment of (bio)molecules containing primary amines, -NH2.

You can use them for:

  • Biosensors and Immunoassays
  • Anti-bacterial
  • Conductors in electronic chips
  • Metal-enhanced fluorescence (MEF)
  • SERS

We commercialize high quality Gold nanostars, formed by multiple branches with sharp tips with highly-competitive characteristics guarantying to meet specifications.

TEM Microscopy

image020 image021

Our Gold nanostars are available in size 40 nm but can be produced in other sizes upon request:

  • PVP STABILIZED: Polyvinylpyrollidone (PVP) is a polymer that binds strongly to the gold nanoparticle surface providing greater stability than citrate or tannic acid.
  • SILICA COATED: silica coating confers thermodynamic and colloidal stability to gold nanostars. 

You can use them for:

  • Surface Enhanced Raman Scattering (SERS)
  • Biosensors

We offer you one of the smallest possible gold probes (0.8nm – 3nm), ideal for ultra-high-resolution EM applications. They have been extensive characterized (TEM, UV-Visible spectroscopy and DLS data), demonstrating a narrow size distribution.

TEM Microscopy

 image012 image010

Intrinsic fluorescence

image016image017

 

Our Gold nanoclusters are available in sizes ranging from 0.8 nm to 3 nm with different surface functionalities in a variety of solvent compositions.

  • CARBOXILATED :can be activated by EDC and NHS to form an intermediate that can subsequently react with primary amine groups on a specific protein or any other ligand to be conjugated
  • THIOPRONINE :
  • AMINE: prepared for binding to the negative charges of DNA backbone

Customer conjugation is available upon request.

You can use them for:

  • Probes for molecular biology or electron microscopy
  • Antibody labelling
  • Bioelectrochemistry
  • Biosensors

It is commonly known that the properties of Gold nanoparticles depend strongly upon their size and shape. Our Gold nanoparticles are exhaustive characterized (TEM, DLS, UV-Vis, Z-potential measurements), possess high monodispersity (CV<15%) and great stability.

Our Gold nanoparticles are available in sizes ranging from 3 nm to 100 nm in diameter with different surface functionalities in a variety of solvent compositions.

CITRATE STABILIZED:

Attachment of (bio)molecules containing thiols groups –SH.

COOH-PEG:

Covalent attachment of (bio)molecules containing primary amines, -NH2.

LIPOIC ACID:

Covalent attachment of (bio)molecules containing primary amines, -NH2.

SILICA COATED:

Silica coating confers thermodynamic and colloidal stability to gold nanoparticles. It also increases the surface area for conjugation of biomolecules, or, as a porous material, can be loaded with drugs, dye molecules, or other imaging agents either via physical adsorption or covalent attachment. Lastly, the silica-coating on gold nanoparticles can enhance imaging contrast by more than 3 fold.

Aplications

> Diagnostics-Immunoassays
> Biosensors
> Probes for molecular biology or electron microscopy
> Drug delivery
> Catalyst in chemical reactions
> Conductors in electronic chips

CAT# PRODUCT NAME SIZE PRICE/ €
Custom Silica Coated Gold Nanoparticles size from 10 to 200nm

3 mg

720,00
Custom Silica Coated Gold Nanoparticles size from 10 to 200nm

10 mg

2.000,00
Custom Silica coated Gold nanostars

3 mg

915,00
Custom Silica coated Gold nanostars

10 mg

2.520,00